Transamidinase of Hog Kidney
نویسندگان
چکیده
منابع مشابه
Hog kidney gluconokinase.
Previous studies (1, 2) have indicated that gluconate is extensively metabolized in the mammal without, prior conversion to glucose. The int,erpretation of experiments performed with labeled gluconate has rested upon the assumption (3) that gluconate is converted to 6-phosphogluconate in t,he course of its metabolism. The enzyme, gluconokinase, which catalyzes this reaction has been partially p...
متن کاملStudies of hog kidney acylase I. I. Comparison with hog kidney dialkylfluorophosphatase.
Hog kidney Acylase I, an enzyme which hydrolyzes N-acetylL-amino acids, has been purified approximately 40-fold by Greenstein (1). During the course of the purification of hog kidney dialkylfluorophosphatase, it was consistently found that the protein fractions containing DFPasel activity also possessed very high Acylase I activity. Inasmuch as the DFPase and Acylase I activities were found to ...
متن کاملSome factors affecting kidney transamidinase activity in rats.
Although it is well established that only two enzymes, transamidinase and guanidoacetate methylpherase, are directly involved in the formation of creatine (l-4), almost nothing is known about the regulation of creatine synthesis. Since various diseases alter creatine metabolism (5), it is possible that a study of transamidinase and guanidoacetate methylpherase activities in some of these diseas...
متن کاملFluorescence properties of hog kidney aminoacylase I.
The state of the tryptophan residues of porcine kidney aminoacylase I (EC 3.5.1.14) was investigated by fluorescence spectroscopy and chemical modification. The pH-dependence of the fluorescence emission spectrum of the enzyme indicates that its native conformation prevails between pH 6 and 9.5. Within this range, the ionization of a residue with an apparent pKa of 7.1 quenches the enzyme fluor...
متن کاملPurification of hog kidney renin with immobilized monoclonal antirenin.
Spleen cells from mice immunized with partially purified hog kidney renin were fused with mouse myeloma cells to produce a stable monoclonal hybridoma cell line that synthesizes an antibody against renin. A single monoclonal antibody was chosen for study and has been produced in large quantity and purified by affinity chromatography on protein A-Sepharose. The antirenin, which belongs to the Ig...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96580-4